MG Cusi… - Expert Review of Anti-infective …, 2011 - ingentaconnect.com ... Other methods, including in vitro and in vivo viral isolation, the plaque-reduction neu- tralization test (PRNT) [15,36,37], immunoblot- ting (IB) [38–40], hema gglutination assay (HA) and the complement fixation test can be per- formed, but their low sensitivity and the need for ...