M Gonzalez-Aparicio, I Mauleon, P Alzuguren… - Gene Therapy, 2011 - nature.com Standard methods for producing high-capacity adenoviral vectors (HC-Ads) are based on co-infection with a helper adenovirus (HV). To avoid HV encapsidation, its packaging signal (Ψ) is flanked by recognition sequences for recombinases expressed in the producing ... All 3 versions
H Cao, G Zhang… - Brain Research, 2011 - Elsevier Because of the numerous types of neurons in the brain, and particularly the forebrain, neuron type-specific expression will benefit many potential applications of direct gene transfer. The two most promising approaches for achieving neuron type-specific ...
PJ Ross, MA Kennedy, C Christou… - Journal of …, 2011 - Am Soc Microbiol ... hdAd-PGK-mSEAP, hdAd-lacZ, and hdAd 28E4 were amplified with the helpervirus AdNG163 (46) or AdJR46 using 116 cells and standard techniques (46, 49). ... Consistent with this, we also observed an increase in hexon at 8 hpi in cells infected with the helpervirus. ... Related articles - All 3 versions
RM Kotin - Human Molecular Genetics, 2011 - Oxford Univ Press ... The AAV rep and cap genes, vg and helpervirus functions are introduced efficiently into Sf9 cells by baculovirus infection. ... S.,; Elliger C.,; Podsakoff G.,; Villarreal L.,; Kurtzman GJ,; Iwaki Y.,; Colosi P. Adeno-associated virus vectors can be efficiently produced without helpervirus. Related articles - All 3 versions
DJ Palmer… - Cold Spring Harbor protocols, 2011 - ncbi.nlm.nih.gov ... Palmer DJ, Ng P. INTRODUCTION This protocol describes the characterization of helper-dependent adenoviral vectors (HDAd) with respect to physical titer, helpervirus contamination, and genomic structure using standard molecular biology techniques. ...
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